不同表面修饰的石墨烯量子点对拟南芥幼苗毒性机制的研究

With the wide application of nanomaterials, artificial nanomaterials have become a new environmental pollutant. Plants are one of the key objects threatened by the pollution of artificial nanomaterials. However, for the same type of artificial nanomaterials, some studies have shown that it can inhibit the growth of plants, while some studies have shown that it does not affect or even promote the growth of plants. Even for this phenomenon, there is a lack of systematic and in-depth study on it’s causes, so cannot fundamentally objective evaluation of the damage artificial nanomaterials to the plant. The surface modification of nanomaterials is probably the important reason that affects the interaction between nanomaterials and plants, and then affects the toxicity effect of nanomaterials on plants. In this study, graphene quantum dots were used as typical artificial nanomaterials , modified three different types of surface materials (positive, negative and near neutral respectively) to study their distribution, growth, changes in physiological and biochemical indexes and the resulting protein expression (proteomics) differences in Arabidopsis seedlings, in order to clarify the toxicity mechanism of different surface modification of nanomaterials on plants from different levels, we could provide a more reliable scientific basis for the safety evaluation of plant toxicity of nanomaterials, and provide a new idea for the study of plant hazard prevention and control of artificial nanomaterials.

随着纳米材料的广泛应用,人工纳米材料已成为一种新型的环境污染物。植物是受到人工纳米材料污染威胁的重点对象之一，但对于同种类型的人工纳米材料，有的研究表明会抑制植物的生长，而有的研究却表明并不影响甚至能够促进植株的生长，对这种截然不同的现象，其原因目前还缺乏系统深入的研究，因此无法从根本上客观评价人工纳米材料对植物造成的危害。而纳米材料的不同表面修饰很可能是影响纳米材料与植物间的相互作用，进而影响纳米材料对植物毒性效应的重要原因。本研究拟将石墨烯量子点作为典型人工纳米材料，分别修饰3种不同类型的表面材料（分别呈正电性、负电性和近电中性），研究其在拟南芥幼苗体内的分布状况、生长和生理生化指标的变化规律及其导致的蛋白质表达(蛋白质组学) 差异，以期从不同水平上阐明纳米材料不同表面修饰对植物的毒性机制，为纳米材料植物毒性安全评价提供更可靠的科学依据，为研究人工纳米材料的植物危害防治提供新思路。